{"id":4741,"date":"2017-11-13T15:54:55","date_gmt":"2017-11-13T21:54:55","guid":{"rendered":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/?page_id=4741"},"modified":"2024-08-26T08:58:52","modified_gmt":"2024-08-26T13:58:52","slug":"required-sto-biosafety-practices","status":"publish","type":"page","link":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/required-sto-biosafety-practices\/","title":{"rendered":"STO Biosafety: Required Work Practices"},"content":{"rendered":"<div data-modular-content-collection><h6>Required Work Practices:<\/h6>\n<p>(Adopted from\u00a0BMBL (Section IV)\u00a0and the University of Minnesota)<\/p>\n<span class=\"collapseomatic arrowleft\" id=\"id69e36b3623306\"  tabindex=\"0\" title=\"&lt;strong&gt;BIOSAFETY LEVEL 1: Description &amp; Work Practices&lt;\/strong&gt;\"    ><strong>BIOSAFETY LEVEL 1: Description &amp; Work Practices<\/strong><\/span><div id=\"target-id69e36b3623306\" class=\"collapseomatic_content \">\n<ul>\n<li>Suitable for work involving:\n<ul>\n<li>Well-characterized agents not known to consistently cause disease in immunocompetent adult humans, and that present minimal potential hazard to laboratory personnel and the environment.<\/li>\n<li>Transgenic or wild-type laboratory animals (e.g., rodents) that are both:\n<ul>\n<li>Free of zoonotic diseases.<\/li>\n<li>Not infected with, implanted with, or containing RG2 or higher agents or materials.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<li>Work is typically conducted on open bench tops using standard microbiological practices.<\/li>\n<li>Laboratory personnel must have specific training in the procedures conducted in the laboratory and must be supervised by a scientist with training in microbiology or a related science.<\/li>\n<li>Personal health status may impact an individual\u2019s susceptibility to infection, ability to receive immunizations or prophylactic interventions. \u00a0Individuals having these conditions should be encouraged to self-identify to the institution\u2019s healthcare provider for appropriate counseling and guidance.<\/li>\n<\/ul>\n<ul>\n<li><strong>Standard Microbiological Practices<\/strong>\n<ul>\n<li>Wash hands after handling viable materials, after removing gloves, and before leaving the laboratory.<\/li>\n<li>Wear pants (or other clothing that covers legs) and close-toed shoes.<\/li>\n<li><strong>Lab Coats.<\/strong>\u00a0 Are generally not necessary. \u00a0However, wear lab coat or other protective clothing when handling viable materials; remove protective clothing before leaving lab areas. \u00a0All protective clothing must be either disposed of in the laboratory or laundered by the work unit, it should never be taken home.<\/li>\n<li><strong>Gloves.<\/strong> Wear gloves whenever contact with microorganisms could be reasonably anticipated and\/or whenever skin on hands is not intact \u2013 including if a rash is present. \u00a0Change gloves when contaminated, glove integrity is compromised\/suspect, or when otherwise necessary.<\/li>\n<li><strong>Goggles.<\/strong> \u00a0Wear chemical splash goggles\u00a0when conducting procedures that have the potential to create splashes of microorganisms or other hazardous materials.<\/li>\n<li>Keep laboratory doors closed; only individuals who are involved with the work are allowed in the area.<\/li>\n<li>Mouth pipetting is prohibited; mechanical pipetting devices must be used.<\/li>\n<li>Perform all procedures with a focus on\u00a0minimizing the creation of splashes and\/or aerosols.<\/li>\n<li>Food, eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption are not\u00a0permitted in laboratory areas (you don&#8217;t even want to be chewing gum in the lab!).<\/li>\n<li>Pay attention to hand and mouth hygiene while working in the lab (e.g., do not put a pen in you mouth, or behind your ear).<\/li>\n<li>Plants and animals not associated with the work being performed should not be permitted in the laboratory.<\/li>\n<li><strong>Sharps<\/strong>. \u00a0Follow the policies and procedures\u00a0on the <a href=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/sharps-safety\/\" target=\"_blank\" rel=\"noopener noreferrer\">Sharps Safety page<\/a>, including:\n<ul>\n<li>Provide sharps containers within easy reach of work stations.<\/li>\n<li>Laboratory supervisors should adopt improved engineering and work practice controls that reduce risk of sharps injuries.<\/li>\n<li>Needles must not be bent, sheared, broken, recapped, removed from disposable syringes, or otherwise manipulated by hand before disposal.<\/li>\n<li>Broken glassware must not be handled directly. \u00a0Instead, it must be removed using a brush and dustpan, tongs, or forceps. \u00a0Plastic ware should be substituted for glassware whenever possible.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Decontaminate:\u00a0<\/strong>\n<ul>\n<li>Work surfaces after completion of work and after any spill or splash of potentially infectious material with appropriate disinfectant.<\/li>\n<li>All cultures, stocks, and other potentially infectious materials before disposal using an effective method. \u00a0Materials to be decontaminated outside of the immediate laboratory must be placed in a durable, leak proof container and secured for transport.<\/li>\n<li>Follow the appropriate\u00a0<a href=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/biological-spills-decontamination-clean-up\/\" target=\"_blank\" rel=\"noopener noreferrer\">decontamination procedures<\/a>.<\/li>\n<\/ul>\n<\/li>\n<li>A sign incorporating the universal biohazard symbol must be posted at the entrance to the laboratory when infectious agents are present. \u00a0The sign must include the name and phone number of the laboratory supervisor or other responsible personnel, and may include the name of the agent(s) in use.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<p><br class=\"none\" \/><span class=\"collapseomatic arrowleft\" id=\"id69e36b36233ef\"  tabindex=\"0\" title=\"&lt;strong&gt;BIOSAFETY LEVEL 2: Description &amp; Work Practices&lt;\/strong&gt;\"    ><strong>BIOSAFETY LEVEL 2: Description &amp; Work Practices<\/strong><\/span><div id=\"target-id69e36b36233ef\" class=\"collapseomatic_content \">\n<ul>\n<li>Suitable for work involving agents that pose moderate hazards to personnel and the environment. \u00a0<em>It is more restrictive than BSL-1. \u00a0It includes\u00a0all\u00a0BSL-1 practices plus the following:<\/em>\n<ul>\n<li>All persons entering the laboratory must be advised of the potential hazards and meet specific entry\/exit requirements.<\/li>\n<li>Laboratory personnel have specific training in handling pathogenic agents and are supervised by scientists competent in handling infectious agents and associated procedures. \u00a0The laboratory supervisor must ensure that laboratory personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-2 agents.<\/li>\n<li>Access to the laboratory is restricted when work is being conducted.<\/li>\n<li><strong>Protective Laboratory Coats<\/strong>, gowns, smocks, or uniforms designated for laboratory use must be worn while working with hazardous materials. \u00a0Remove protective clothing before leaving the laboratory. \u00a0Dispose of protective clothing appropriately, or deposit it for laundering by the institution. \u00a0It is recommended that laboratory clothing not be taken home.<\/li>\n<li><strong>Gloves<\/strong> must be worn to protect hands from exposure to hazardous materials. \u00a0Glove selection should be based on an appropriate risk assessment. \u00a0Gloves must not be worn outside the laboratory. \u00a0In addition, BSL-2 laboratory workers should:\n<ul>\n<li>Change gloves when contaminated, glove integrity is compromised, or when otherwise necessary.<\/li>\n<li>Remove gloves and wash hands when work with hazardous materials has been completed and before leaving the laboratory.<\/li>\n<li>Do not wash or reuse disposable gloves. \u00a0Dispose of used gloves with other contaminated laboratory waste. \u00a0Hand washing protocols must be rigorously followed.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Biosafety Cabinet. \u00a0<\/strong>All procedures in which infectious aerosols or splashes may be created are conducted in Biosafety Cabinets\u00a0or other physical containment equipment. \u00a0These may include pipetting, centrifuging, grinding, blending, shaking, mixing, sonicating, opening containers of infectious materials, inoculating animals intranasally, and harvesting infected tissues from animals or eggs.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<hr \/>\n<h6>Additional Resources:<\/h6>\n<ul>\n<li><strong><a href=\"https:\/\/www.cdc.gov\/labs\/pdf\/SF__19_308133-A_BMBL6_00-BOOK-WEB-final-3.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Biosafety in Microbial and Biomedical Laboratories (BMBL) 6th Edition<\/a> <a href=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/files\/2024\/07\/BMBL6Cover.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" data-attachment-id=\"5818\" data-permalink=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/required-sto-biosafety-practices\/bmbl6cover-2\/\" data-orig-file=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/files\/2024\/07\/BMBL6Cover.jpg\" data-orig-size=\"1200,1800\" data-comments-opened=\"0\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"BMBL6Cover\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/files\/2024\/07\/BMBL6Cover-683x1024.jpg\" class=\"alignright wp-image-5818\" src=\"https:\/\/wp.stolaf.edu\/chemical-hygiene\/files\/2024\/07\/BMBL6Cover-200x300.jpg\" alt=\"\" width=\"109\" height=\"164\" \/><\/a><\/strong> (<em>The BMBL is both a code of practice and an authoritative reference. \u00a0Knowledge sufficient to work safely with hazardous microorganisms requires a careful review of the entire BMBL. \u00a0This will offer the reader an understanding of the biosafety principles\u00a0that serve as the basis for the concepts and recommendations included in this reference. \u00a0Reading only selected sections will not adequately prepare even an experienced laboratory worker to handle potentially infectious agents safely<\/em>).<\/li>\n<\/ul>\n<h6><\/h6>\n<ul>\n<li><a href=\"http:\/\/www.asm.org\/images\/asm_biosafety_guidelines-FINAL.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>American Society for Microbiology:\u00a0<\/strong><strong>Guidelines for Biosafety Teaching in Laboratories (2012)<\/strong><\/a>\n<ul>\n<li><strong><a href=\"http:\/\/www.asm.org\/images\/Education\/FINAL_Biosafety_Guidelines_Appendix_Only.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Appendix to ASM Guidelines\u00a0(2012)<\/a><\/strong><\/li>\n<li><strong><a href=\"http:\/\/facultyprograms.org\/images\/Moocs\/Biosafety%20Resources.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Frequently Asked Questions<\/a><\/strong><\/li>\n<\/ul>\n<\/li>\n<li><strong><a href=\"http:\/\/asmscience.org\/content\/education\/protocol\" target=\"_blank\" rel=\"noopener noreferrer\">American Society for Microbiology: Laboratory Protocols<\/a><\/strong><\/li>\n<li><a href=\"http:\/\/www.nap.edu\/openbook.php?isbn=0309039754\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>Biosafety in the Laboratory: Prudent Practices for the Handling and Disposal of Infectious Materials<\/strong><\/a><\/li>\n<li><strong><a href=\"https:\/\/absa.org\/intro\/\" target=\"_blank\" rel=\"noopener\">ABSA: Introduction to Biosafety for Undergraduate and Graduate Students<\/a><\/strong><\/li>\n<li><a href=\"http:\/\/Involving Recombinant or Synthetic Nucleic Acid Molecules\" target=\"_blank\" rel=\"noopener\"><strong>NIH Guidelines for Research\u00a0Involving Recombinant or Synthetic Nucleic Acid Molecules<\/strong><\/a><\/li>\n<li><a href=\"http:\/\/Guiding Principles for Biosafety Governance\" target=\"_blank\" rel=\"noopener\"><strong>Guiding Principles for Biosafety Governance<\/strong><\/a><\/li>\n<\/ul>\n<p><!-- begin-migrated-from-panel-builder --><!-- end-migrated-from-panel-builder --><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Required Work Practices: (Adopted from\u00a0BMBL (Section IV)\u00a0and the University of Minnesota) Additional Resources: Biosafety in Microbial and Biomedical Laboratories (BMBL) 6th Edition (The BMBL is both a code of practice [&hellip;]<\/p>\n","protected":false},"author":156,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-panels-beside-sidebar.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-4741","page","type-page","status-publish","hentry"],"acf":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/pages\/4741","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/users\/156"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/comments?post=4741"}],"version-history":[{"count":15,"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/pages\/4741\/revisions"}],"predecessor-version":[{"id":5839,"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/pages\/4741\/revisions\/5839"}],"wp:attachment":[{"href":"https:\/\/wp.stolaf.edu\/chemical-hygiene\/wp-json\/wp\/v2\/media?parent=4741"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}